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KMID : 0375319950170010129
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Journal of Clinical Pathology and Quality Control
1995 Volume.17 No. 1 p.129 ~ p.132
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Change of Blood Ammonia Levels with time Lapse After Collection
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Abstract
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Background:
We determined blood ammonia level by the direct colorimetric method. This method requires complicated technique and are time consuming. The measure of blood ammonia level must be completed quickly, since ammonia concentration increases with
lapse of
time after blood collection. A simple and rapid method is required for determining blood ammonia level.
Methods:
Blood ammonia concentration was determined both by the Ammonia Checker II and by the direct colorimetric method using whole blood from 30 within 10 mints after collection. We determined blood ammonia levels by the Ammonia Checker II after 1
hour and
2 hour storage at room temperature.
Results:
Blood ammonia values obtained by the Ammonia Checker II were in good correlation with those by the direct colorimetric method (r=0.98. y=1.02x-4.2430). The average increments of blood ammonia concentration were 23.0% at 1 hour and 40.9% at 2
hours
comparing with the results determined within 10 minutes after collection.
Conclusions:
Ammonia Checker II has distinct advantages for blood ammonia determination in respect to its simplicity, rapidity, and small sample volume.
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